Note that the width of the ISO rectangles does not factor in the 1% accuracy of the pipette Checkit ®.Ĭan the Checkits be used to test someone’s pipetting technique? The rectangle illustrates the acceptable range. ISO 8655 standard covers the accuracy requirements of hand held, single channel, adjustable pipettes. What is the cross-hatched rectangle on the Checkit Pro? We will likely be able to substantiate 1% accuracy once the product has enough history.
The Checkit Pro is accurate to within 1%, aside from external influences such as evaporation. If your hands are shaky, it helps to use two hands when pipetting or ask someone else for assistance. You can also use your pipette tip, carefully, to help guide dye to center after pipetting. On the smaller volume wells, you can feel a divot at the bottom to guide the pipette tip. You can touch your pipette tip to the bottom of the well to help find the center. How do I center the dye in the well of a Checkit Pro? Leaving the tab up will reduce evaporation, thereby providing you more time to compare the meniscus to the adjacent markings.įirst when dispensing into the well, if you do a blowout, lift the end of the pipette tip out of the pool of dye in the well. Do not push hard, or you will risk breaking the end of the capillary. How hard do I need to push on the well tab? How far?Ī light touch is all that is needed to lift the tab, until you feel it stop moving. To minimize evaporation losses, lift each tab immediately after dispensing dye into its well(s). Lift the well tab at a reasonably quick, comfortable rate, about as quickly as you would turn off or turn on a light switch. How rapidly should the well tabs be lifted up?
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